Am J Stem Cell 2012;1(3):174-181

Original Article
Experimental model for coadjuvant treatment with mesenchymal stem cells for
aortic aneurysm

Luis Riera del Moral, Carlota Largo Aramburu, José Ramón Ramírez García, Luis Riera de Cubas, Damián García-Olmo, Mariano

Department of Angiology and Vascular Surgery, La Paz University Hospital. Madrid, Spain; Experimental Surgery Unit, La Paz University
Hospital- Health Institute Research La Paz (IdiPAZ). Madrid. Spain; Department of Pathology, University Hospital Of Tajo, Aranjuez,
Madrid. Spain; Cell Therapy Laboratory, Investigation Unit. La Paz University Hospital- Health Institute Research La Paz (IdiPAZ). Madrid,
Spain; Department of Surgery, School of Medicine. Autonomous University of Madrid, Spain

Received February 18, 2012; Accepted October 3, 2012; Epub November 30, 2012; Published December 10, 2012

Abstract: Many factors are possibly involved in the inflammatory process which causes the degeneration of the arterial wall in the
formation of Abdominal Aortic Aneurysms. During the last years different experimental models have been published to treat this fault of
the arterial walls. Parallel the clinical treatment has evolved. With this work we have tried to develop an animal model basing on the
clinical current treatment. Finally, we propose a treatment based on mesenchymal cells to disable local immune response, preventing
excessive fibrosis, apoptosis, and inducing intrinsic cellular progenitors. Objective: To present a reproducible superior animal model of
experimentation, intending to show that mesenchymal stem cells inserted in the sac of an artificial aneurysm are able to survive, so that
they can be made accountable for a subsequent beneficial effect upon this condition. Methods: Six Landrace-White pigs, weighing
around 25Kg. We generate 2 aneurysms of abdominal aorta (2x1cm) with Dacron’s patches. Later we treat the aneurysms endoscopic
with a covered endograft. Finally, in one of the aneurysmal sac we introduce 1cc fibrin sealant and in another 1 cc of fibrin sealant with 10
million MSC. Animals were sacrificed at 24 hs and 1, 3, 5, 7 and 9 weeks. Samples of aneurysms were processed histologically (H&E
and Masson). The injected cells were located by immunofluorescence (GFP market). Results: The surgical technique is reproducible
and similar to those conducted in common clinical practice. Histological cross-section samples of cases treated with MSC and analyzed
by a blind researcher present a lower inflammation reaction, or with longer evolution time than in controls. Immunofluorescence studies
have detected cells marked with GFP up to three weeks after treatment. Conclusion: This reproducible animal model is similar to
common clinical treatment. MSC can stand alive at least for three weeks since their implantation within an aneurysm sac. This may
improve the results of conventional endovascular treatment by the stabilization of the aneurysmal sac. (AJSC1202003).

Keywords: Aortic aneurysm, experimental model, adipose derived stem cells

Address all correspondence to:
Dr. Riera del Moral
Vascular Surgery, Department of Surgery
Hospital Universitario La Paz, Madrid, Spain.
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