Am J Stem Cell 2013;2(1):39-51

Review Article
Genetic instability of modified stem cells – a first step towards malignant
transformation?

Doris Steinemann, Gudrun Göhring, Brigitte Schlegelberger

Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany

Received December 20, 2012; Accepted February 8, 2013; Epub March 8, 2013; Published March 18, 2013

Abstract: Induced pluripotent stem cells (iPSC) are important tools in regenerative medicine. Yet, it is becoming increasingly clear that
the reprogramming process, including retroviral transduction with potent oncogenes like c-Myc and long-term cultivation, may induce
genetic instability. Genetically altered iPS cells can grow out and dominate the cell culture. This review intends to comprehensively
summarize the current knowledge on genetic instability of embryonic and iPSCs, with an emphasis on cytogenetic alterations, and
compares these data with what is known from tumorigenesis. (AJSC1212002).

Keywords: Induced pluripotent stem cells, gene therapy, chromosomal instability, genetic integrity, tumor, leukemia, clonal evolution,
chro-mosome, mutation, P53

Address correspondence to: Prof. Brigitte Schlegelberger, Institute of Cell and Molecular Pathology, Hannover Medical School, Carl-
Neuberg-Str. 1, 30625 Hannover, Germany. Tel: +49-511-532-4522; Fax: +49-511-532-4521; E-mail: schlegelberger.brigitte@mh-
hannover.de
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